{"id":14227,"date":"2024-12-04T13:35:31","date_gmt":"2024-12-04T18:35:31","guid":{"rendered":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/?p=14227"},"modified":"2024-12-04T13:35:31","modified_gmt":"2024-12-04T18:35:31","slug":"agp-files-no-longer-accepted-genome-submissions","status":"publish","type":"post","link":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/","title":{"rendered":"AGP Files Will No Longer be Accepted for Genome Submissions"},"content":{"rendered":"<h3><em>Effective March 2025\u00a0<\/em><\/h3>\n<p><span data-contrast=\"none\">Do you submit genomes to NCBI&#8217;s <\/span><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/genbank\/?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=genbank-agp-20241203\"><span data-contrast=\"none\">GenBank<\/span><\/a><span data-contrast=\"none\">? Beginning March 2025, GenBank will no longer accept AGP files for genome submissions. Historically, AGP files were submitted along with contigs as necessary information for constructing assemblies. However, thanks to technology improvements, more and more whole genome shotgun (WGS) sequences submitted to NCBI are gapped assemblies (assemblies with inserted Ns for gaps). <\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><!--more--><\/p>\n<h5><b><span data-contrast=\"none\">How to submit<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/h5>\n<p><span data-contrast=\"none\">Instead of providing contigs and accompanying AGP file(s), please prepare your WGS submission as a gapped assembly. The submission portal user interface for WGS submission will have fewer questions and will be more streamlined. <\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<h5><b><span data-contrast=\"none\">Learn more<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/h5>\n<p><span data-contrast=\"none\">For more information, visit our <\/span><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/genbank\/genomesubmit\/?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=genbank-agp-20241203\"><span data-contrast=\"none\">GenBank Genome Submission guide<\/span><\/a><span data-contrast=\"none\">.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<h5><b><span data-contrast=\"none\">Questions?\u00a0<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/h5>\n<p><span data-contrast=\"none\">Please\u202f<a href=\"mailto:genomes@ncbi.nlm.nih.gov\">reach out to us<\/a>\u202fif you have questions or would like to provide feedback. <\/span><\/p>\n<h5><b><span data-contrast=\"none\">Stay up to date\u00a0<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/h5>\n<p><span data-contrast=\"none\">Follow us on social\u202f<a href=\"https:\/\/x.com\/ncbi\">@NCBI<\/a>\u202fand\u202fjoin our mailing list\u202fto keep up to date with\u202fGenBank and other NCBI news.\u202f\u202f\u00a0<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335557856&quot;:16777215,&quot;335559738&quot;:150,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Effective March 2025\u00a0 Do you submit genomes to NCBI&#8217;s GenBank? Beginning March 2025, GenBank will no longer accept AGP files for genome submissions. Historically, AGP files were submitted along with contigs as necessary information for constructing assemblies. However, thanks to technology improvements, more and more whole genome shotgun (WGS) sequences submitted to NCBI are gapped &hellip; <a href=\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">AGP Files Will No Longer be Accepted for Genome Submissions<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":42204617,"featured_media":14232,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"AGP Files Will No Longer be Accepted for Genome Submissions","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[2248],"tags":[1201719],"table_tags":[],"class_list":["post-14227","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-whats-new","tag-genbank"],"jetpack_publicize_connections":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>AGP Files Will No Longer be Accepted for Genome Submissions - NCBI Insights<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"AGP Files Will No Longer be Accepted for Genome Submissions - NCBI Insights\" \/>\n<meta property=\"og:description\" content=\"Effective March 2025\u00a0 Do you submit genomes to NCBI&#8217;s GenBank? Beginning March 2025, GenBank will no longer accept AGP files for genome submissions. Historically, AGP files were submitted along with contigs as necessary information for constructing assemblies. However, thanks to technology improvements, more and more whole genome shotgun (WGS) sequences submitted to NCBI are gapped &hellip; Continue reading AGP Files Will No Longer be Accepted for Genome Submissions &rarr;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\" \/>\n<meta property=\"og:site_name\" content=\"NCBI Insights\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ncbi.nlm\" \/>\n<meta property=\"article:published_time\" content=\"2024-12-04T18:35:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1100\" \/>\n\t<meta property=\"og:image:height\" content=\"575\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"NCBI Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@NCBI\" \/>\n<meta name=\"twitter:site\" content=\"@NCBI\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"NCBI Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\"},\"author\":{\"name\":\"NCBI Staff\",\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#\/schema\/person\/4f41ea6a31cea38a9a1b1419c048e50b\"},\"headline\":\"AGP Files Will No Longer be Accepted for Genome Submissions\",\"datePublished\":\"2024-12-04T18:35:31+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\"},\"wordCount\":161,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#organization\"},\"image\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1\",\"keywords\":[\"GenBank\"],\"articleSection\":[\"What's New\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\",\"url\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\",\"name\":\"AGP Files Will No Longer be Accepted for Genome Submissions - NCBI Insights\",\"isPartOf\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1\",\"datePublished\":\"2024-12-04T18:35:31+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage\",\"url\":\"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1\",\"contentUrl\":\"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1\",\"width\":1100,\"height\":575,\"caption\":\"Background has digital paper graphics in dark blue and black. 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However, thanks to technology improvements, more and more whole genome shotgun (WGS) sequences submitted to NCBI are gapped &hellip; Continue reading AGP Files Will No Longer be Accepted for Genome Submissions &rarr;","og_url":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/","og_site_name":"NCBI Insights","article_publisher":"https:\/\/www.facebook.com\/ncbi.nlm","article_published_time":"2024-12-04T18:35:31+00:00","og_image":[{"width":1100,"height":575,"url":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png","type":"image\/png"}],"author":"NCBI Staff","twitter_card":"summary_large_image","twitter_creator":"@NCBI","twitter_site":"@NCBI","twitter_misc":{"Written by":"NCBI Staff","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#article","isPartOf":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/"},"author":{"name":"NCBI Staff","@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#\/schema\/person\/4f41ea6a31cea38a9a1b1419c048e50b"},"headline":"AGP Files Will No Longer be Accepted for Genome Submissions","datePublished":"2024-12-04T18:35:31+00:00","mainEntityOfPage":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/"},"wordCount":161,"commentCount":0,"publisher":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#organization"},"image":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage"},"thumbnailUrl":"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1","keywords":["GenBank"],"articleSection":["What's New"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/","url":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/","name":"AGP Files Will No Longer be Accepted for Genome Submissions - NCBI Insights","isPartOf":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/#website"},"primaryImageOfPage":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage"},"image":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage"},"thumbnailUrl":"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1","datePublished":"2024-12-04T18:35:31+00:00","breadcrumb":{"@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ncbiinsights.ncbi.nlm.nih.gov\/2024\/12\/04\/agp-files-no-longer-accepted-genome-submissions\/#primaryimage","url":"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1","contentUrl":"https:\/\/i0.wp.com\/ncbiinsights.ncbi.nlm.nih.gov\/wp-content\/uploads\/2024\/12\/AGP_Files_Blog-e1733176177608.png?fit=1100%2C575&ssl=1","width":1100,"height":575,"caption":"Background has digital paper graphics in dark blue and black. 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