Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated using miRNeasy kit (Qiagen) following the manufacture's protocol, 100ng of total RNA was converted to cDNA and amplified using NuGen V2 RNA-Seq kit (NuGen, San Carlos, CA) Sonication of cDNA was performed to produce an average fragment size of 280 bps and Illumina sequencing adapters were ligated to 500ng of cDNA using NEBNext® mRNA Library Prep Reagent Set (New England Biolabs, Ipswich, MA)