Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Introduction

In this study we used RNA-seq to identify the expression profile of banana plantules in response to two distinct species of plant growth-promoting rhizobacteria, Pseudomonas fluorescens PS006 and Bacillus amyloliquefaciens BS006, in three post-inoculation times: 1 h, 48 h, and 96 h. We investigated differential gene expression for the treatment with the two rhizobacteria.

Gene Ontology enrichment analysis have showed that some categories have similar genes expressed in the two rhizobacteria, despite of and earlier response triggered in Pseudomonas (48 h) compared to Bacillus (96). In addition, Pseudomonas and Bacillus also trigger distinct responses, as such as cellular proliferation for the former and production of energy for the latter. Based on our findings we suggest the application of both rhizobacteria as inoculants since they have complementary effects on the plant response.

Publications

  1. Gamez R.M., Rodríguez F., Vidal N., Ramirez S., Vera R., Landsman D., Mariño-Ramírez L.
    Banana (Musa acuminata) transcriptome profiling in response to rhizobacteria: Bacillus amyloliquefaciens Bs006 and Pseudomonas fluorescens Ps006.
    BMC Genomics. 2019 May 14;20(1):378. doi: 10.1186/s12864-019-5763-5.
  2. Gamez RM, Rodríguez F, Ramírez S, Gómez Y, Agarwala R, Landsman D, Mariño-Ramírez L.
    Genome Sequence of the Banana Plant Growth-Promoting Rhizobacterium Pseudomonas fluorescens PS006.
    Genome Announc. 2016 May 5;4(3). pii: e00329-16. doi: 10.1128/genomeA.00329-16.
  3. Gamez RM, Rodríguez F, Bernal JF, Agarwala R, Landsman D, Mariño-Ramírez L.
    Genome Sequence of the Banana Plant Growth-Promoting Rhizobacterium Bacillus amyloliquefaciens BS006.
    Genome Announc. 2015 Nov 25;3(6). pii: e01391-15. doi: 10.1128/genomeA.01391-15.

Effect of PGPR on banana growth

The effect of Bacillus amyloliquefaciens and Pseudomonas fluorescens inoculation on plant growth was investigated in banana plantules. Eight variables were evaluated in plantules 55 days old: height (cm), leaf area (cm2), pseudostem tickness (mm), total fresh weight (g/plant), root fresh weight (g/plant), shoot fresh weight (g/plant), total dry weight (g/plant), and shoot dry weight (g/plant).

Biological Assay

All variables were significantly higher in samples inoculated with B. amyloliquefaciens or P. fluorescens compared to the control (no PGPR inoculated).

Height
Root fresh weight
Height: control (left), BS0006 (right)
Root fresh weight: control (left), BS0006 (right)
Control (left), Bacillus amyloliquefaciens (right)
Control (left), Bacillus amyloliquefaciens (right)