Alternative titles; symbols
HGNC Approved Gene Symbol: SLMAP
Cytogenetic location: 3p14.3 Genomic coordinates (GRCh38) : 3:57,756,309-57,930,013 (from NCBI)
Wigle et al. (1997) screened a rabbit heart cDNA expression library with anti-sarcolemmal antibodies and isolated a novel cDNA encoding sarcolemmal associated protein (SLAP). Three alternately spliced isoforms, termed SLAP1, SLAP2, and SLAP3, differing at their 5-prime ends, were identified. The 3 cDNAs predicted proteins of 322, 402, and 639 amino acids, with predicted masses of 37, 46, and 74 kD, respectively. Each of the predicted proteins contained a putative transmembrane domain at its carboxy-terminal tail and 2 potential leucine zipper motifs. SLAP showed extended low-level homology with myosins and other coiled-coil proteins. Northern blot analysis of rabbit tissues revealed 3 mRNAs of 5.9, 4.5, and 3.5 kb, each with a distinct pattern of expression. Immunostaining of rat heart tissue sections demonstrated that SLAP had a reticular pattern of expression in the sarcolemma.
Wigle et al. (1997) isolated a 2.4-kb human SLAP clone from a cardiac cDNA library.
By fluorescence in situ hybridization, Wigle et al. (1997) mapped the human SLAP gene to chromosome 3p21.2-p14.3.
Wigle, J. T., Demchyshyn, L., Pratt, M. A. C., Staines, W. A., Salih, M., Tuana, B. S. Molecular cloning, expression, and chromosomal assignment of sarcolemmal-associated proteins: a family of acidic amphipathic alpha-helical proteins associated with the membrane. J. Biol. Chem. 272: 32384-32394, 1997. [PubMed: 9405447] [Full Text: https://doi.org/10.1074/jbc.272.51.32384]