Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Mar;106(5-6):1799-1811.
doi: 10.1007/s00253-022-11834-0. Epub 2022 Feb 24.

Biotechnological production of bacosides from cell and organ cultures of Bacopa monnieri

Hosakatte Niranjana Murthy  1
Affiliations
Review

Biotechnological production of bacosides from cell and organ cultures of Bacopa monnieri

Hosakatte Niranjana Murthy. Appl Microbiol Biotechnol. 2022 Mar.

Abstract

Bacopa monnieri (L.) Wettst. (BM), also known as 'Brahmi' or 'Water Hyssop', has been utilized as a brain tonic, memory enhancer, sensory organ revitalizer, cardiotonic, anti-anxiety, antidepressant and anticonvulsant agent in the Indian system of medicine Ayurveda for centuries. BM is beneficial in the treatment of Parkinson's disease, Alzheimer's disease, epileptic seizures and schizophrenia in recent pharmacological research. Dammarane-type triterpenoid saponins containing jujubogenin and pseudojujubogenin as aglycones, also known as bacosides, are the principal chemical ingredients identified and described from BM. Bacosides have been shown to have anti-ageing, anticancer, anticonvulsant, antidepressant, anti-emetic, anti-inflammatory and antibacterial properties in a variety of pre-clinical and clinical studies. The pharmaceutical industry's raw material comes from wild sources; nevertheless, the concentration of bacosides varies in different regions of the plants, as well as seasonal and genotypic variation. Cell and tissue cultures are appealing alternatives for the long-term manufacture of bioactive chemicals, and attempts to produce bacosides using in vitro cultures have been made. This review discusses the biotechnological approaches used to produce bacosides, as well as the limitations and future potential. KEY POINTS: • Bacosides extracted from Bacopa monnieri are important pharmaceutical compounds. • The current review provides insight into biotechnological interventions for the production of bacosides using in vitro cultures. • Highlights the prospects improvement of bacoside production through metabolic engineering.

Keywords: Bacopa monnieri; Bioreactor cultures; Cell cultures; Shoot cultures; Triterpenoid saponins.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Ahire ML, Laxmi S, Walunj PR, Kavi Kishor PB, Nikam TD (2014) Effect of potassium chloride and calcium chloride induces stress on in vitro cultures of Bacopa monnieri (L.) Pennell and accumulation of medicinally important bacoside A. J Plant Biochem Biotechnol 23:366–378
    1. Banerjee S, Anand U, Ghosh S, Ray D, Ray P, Nandy S, Deshmukh GD, Tripathi V, Dey A (2021) Bacosides from Bacopa monnieri extract: an overview of the effect on neurological disorders. Phytotherapy Res 35:5668–5679
    1. Bansal M, Reddy MS, Kumar A (2017) Optimization of cell growth and bacoside-A production in suspension cultures of Bacopa monnieri (L.) Wettst. using response surface methodology. In Vitro Cell Dev Biol Plant 53:527–537
    1. Bansal M, Kumar A, Reddy MS (2015) Production of bacoside A, a memory enhancer from hairy root cultures of Bacopa monnieri (L.) Wettst. J Appl Res Med Aromat Plants 2:92–101
    1. Bansal M, Kumar A, Reddy MS (2014) Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside-A’ production from Bacopa monnieri (L.) Wettst. Acta Physiol Plant 36:2793–2801

LinkOut - more resources