Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus
- PMID: 2440680
- DOI: 10.1111/j.1432-1033.1987.tb13547.x
Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus
Abstract
The complete nucleotide sequence of the beta-xylosidase gene (xynB) of Bacillus pumilus IPO and its flanking regions was established. A 1617-bp open reading frame for beta-xylosidase, a homodimer enzyme, was observed. The amino acid sequence of the N-terminal region and the molecular mass 62607 Da) of the beta-xylosidase subunit, deduced from the DNA sequence, agreed with the result obtained with the purified enzyme. The Shine-Dalgarno sequence was found 8 bp upstream of the initiation codon, ATG. The xylanase gene (xynA) of the same strain was 4.6 kbp downstream of the 3' end of xynB, and its DNA sequence was reported in our previous paper [Fukusaki, E., Panbangred, W., Shinmyo, A. & Okada, H. (1984) FEBS Lett. 171, 197-201]. The results of the Northern hybridization suggested that the mRNA of xynA and xynB were produced separately. The 5' and 3' ends of the xynA and xynB gene were mapped with nuclease S1. The '-10' regions for promoter sequences of both genes were similar to the consensus sequence for B. subtilis RNA polymerases, the '-35' regions were different from all the known promoters for B. subtilis RNA polymerases.
Similar articles
-
Sequence and properties of beta-xylosidase from Bacillus pumilus IPO. Contradiction of the previous nucleotide sequence.Eur J Biochem. 1991 Dec 18;202(3):1197-203. doi: 10.1111/j.1432-1033.1991.tb16490.x. Eur J Biochem. 1991. PMID: 1765080
-
Identification and characterization of clustered genes for thermostable xylan-degrading enzymes, beta-xylosidase and xylanase, of Bacillus stearothermophilus 21.Appl Environ Microbiol. 1994 Jul;60(7):2252-8. doi: 10.1128/aem.60.7.2252-2258.1994. Appl Environ Microbiol. 1994. PMID: 8074507 Free PMC article.
-
Cloning of high activity xylanase gene from Bacillus pumilus PJ19.J Biochem Mol Biol Biophys. 2002 Oct;6(5):365-9. doi: 10.1080/1025814021000024307. J Biochem Mol Biol Biophys. 2002. PMID: 12385974
-
Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.Eur J Biochem. 1984 Jan 16;138(2):267-73. doi: 10.1111/j.1432-1033.1984.tb07911.x. Eur J Biochem. 1984. PMID: 6421572
-
Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum".Appl Environ Microbiol. 1990 Apr;56(4):1017-24. doi: 10.1128/aem.56.4.1017-1024.1990. Appl Environ Microbiol. 1990. PMID: 2111111 Free PMC article.
Cited by
-
Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake in Wadi El-Natron.Biotechnol Biotechnol Equip. 2015 Mar 4;29(2):315-323. doi: 10.1080/13102818.2014.995932. Epub 2015 Jan 13. Biotechnol Biotechnol Equip. 2015. PMID: 26019646 Free PMC article.
-
Functional association of catalytic and ancillary modules dictates enzymatic activity in glycoside hydrolase family 43 β-xylosidase.J Biol Chem. 2012 Mar 16;287(12):9213-21. doi: 10.1074/jbc.M111.314286. Epub 2012 Jan 23. J Biol Chem. 2012. PMID: 22270362 Free PMC article.
-
Purification and characterization of an acetyl xylan esterase from Bacillus pumilus.Appl Environ Microbiol. 1998 Feb;64(2):789-92. doi: 10.1128/AEM.64.2.789-792.1998. Appl Environ Microbiol. 1998. PMID: 10215579 Free PMC article.
-
Identification of the Butyrivibrio fibrisolvens xylosidase gene (xylB) coding region and its expression in Escherichia coli.Appl Environ Microbiol. 1989 Feb;55(2):306-11. doi: 10.1128/aem.55.2.306-311.1989. Appl Environ Microbiol. 1989. PMID: 2497707 Free PMC article.
-
Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities.Appl Environ Microbiol. 1991 Apr;57(4):1227-34. doi: 10.1128/aem.57.4.1227-1234.1991. Appl Environ Microbiol. 1991. PMID: 1905520 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
