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. 2012 Oct;44(10):1117-21.
doi: 10.1038/ng.2391. Epub 2012 Aug 26.

Exome sequencing of hepatitis B virus-associated hepatocellular carcinoma

Jian Huang  1 , Qing Deng, Qun Wang, Kun-Yu Li, Ji-Hong Dai, Niu Li, Zhi-Dong Zhu, Bo Zhou, Xiao-Yan Liu, Rui-Fang Liu, Qian-Lan Fei, Hui Chen, Bing Cai, Boping Zhou, Hua-Sheng Xiao, Lun-Xiu Qin, Ze-Guang Han
Affiliations

Exome sequencing of hepatitis B virus-associated hepatocellular carcinoma

Jian Huang et al. Nat Genet. 2012 Oct.

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and shows a propensity to metastasize and infiltrate adjacent and more distant tissues. HCC is associated with multiple risk factors, including hepatitis B virus (HBV) infection, which is especially prevalent in China. Here, we used exome sequencing to identify somatic mutations in ten HBV-positive individuals with HCC with portal vein tumor thromboses (PVTTs), intrahepatic metastases. Both C:G>A:T and T:A>A:T transversions were frequently found among the 331 non-silent mutations. Notably, ARID1A, which encodes a component of the SWI/SNF chromatin remodeling complex, was mutated in 14 of 110 (13%) HBV-associated HCC specimens. We used RNA interference to assess the roles of 91 of the confirmed mutated genes in cellular survival. The results suggest that seven of these genes, including VCAM1 and CDK14, may confer growth and infiltration capacity to HCC cells. This study provides a view of the landscape of somatic mutations that may be implicated in advanced HCC.

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References

    1. N Engl J Med. 2010 Oct 14;363(16):1532-43 - PubMed
    1. Oncogene. 2007 Apr 2;26(15):2166-76 - PubMed
    1. Semin Liver Dis. 2010 Feb;30(1):75-86 - PubMed
    1. Genome Res. 2010 Sep;20(9):1297-303 - PubMed
    1. Am J Hum Genet. 2006 Oct;79(4):759-64 - PubMed

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