Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Jun 1;62(Pt 6):518-21.
doi: 10.1107/S1744309106014862. Epub 2006 May 5.

Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding

Juan Sanchez-Weatherby  1 , Stacey Southall, Arthur Oubrie
Affiliations

Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding

Juan Sanchez-Weatherby et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Effect of seed serial dilution (1/10 to 1/106), successively in (a) to (f), on the crystal growth of the apo-form variant A of sGDH from A. calcoaceticus. The scale bar represents 300 µm.
Figure 2
Figure 2
Crystals of apo forms of sGDH variants A (a), B (b) and G (c) and homologues from E. coli (d) and S. coelicolor (e, f). (e) and (f) show two different views of a S. coelicolor crystal. The scale bar represents 60 µm.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760–­763. - PubMed
    1. Felton, L. M. & Anthony, C. (2005). Nature (London), 433, E10. - PubMed
    1. Igarashi, S., Hirokawa, T. & Sode, K. (2004). Biomol. Eng.21, 81–89. - PubMed
    1. Kasahara, T. & Kato, T. (2003). Nature (London), 422, 832. - PubMed
    1. Kasahara, T. & Kato, T. (2005). Nature (London), 433, E11–E12.

Publication types

MeSH terms

Substances