Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Nov;62(11):4081-5.
doi: 10.1128/aem.62.11.4081-4085.1996.

Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14

O Asaka, M Shoda

Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14

O Asaka et al. Appl Environ Microbiol. 1996 Nov.

Abstract

Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. subtilis RB14-C, a spontaneous streptomycin-resistant mutant of RB14, was used. Damping-off was suppressed when the culture broth, cell suspension, or cell-free culture broth of RB14-C was inoculated into soil. Iturin A and surfactin were recovered from the soils inoculated with the cell suspension of RB14-C, confirming that RB14-C produced them in soil. The gene lpa-14, which was cloned from RB14 and required for the production of both antibiotics, was mutated in RB14-C, and a mutant, R(Delta)1, was constructed. The level of disease suppressibility of R(Delta)1 was low, but R(Delta)1(pC115), a transformant of R(Delta)1 with the plasmid pC115 carrying lpa-14, was restored in suppressibility. These results show that the antibiotics iturin A and surfactin produced by RB14 play a major role in the suppression of damping-off caused by R. solani. RB14-C, R(Delta)1, and R(Delta)1(pC115) persisted in soil during the experimental period and were recovered from the soil, mostly as spores.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Appl Environ Microbiol. 1982 Sep;44(3):708-14 - PubMed
    1. Appl Environ Microbiol. 1994 Jun;60(6):2023-30 - PubMed
    1. Appl Environ Microbiol. 1992 Sep;58(9):2978-82 - PubMed
    1. Appl Environ Microbiol. 1990 Apr;56(4):908-12 - PubMed
    1. Appl Environ Microbiol. 1995 Mar;61(3):849-54 - PubMed

LinkOut - more resources