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. 1999 Jul;20(14):1269-75.
doi: 10.1016/s0142-9612(99)00021-6.

Visualization of in vitro protein-rejecting properties of PEGylated stealth polycyanoacrylate nanoparticles

M T Peracchia  1 , S Harnisch, H Pinto-Alphandary, A Gulik, J C Dedieu, D Desmaële, J d'Angelo, R H Müller, P Couvreur
Affiliations

Visualization of in vitro protein-rejecting properties of PEGylated stealth polycyanoacrylate nanoparticles

M T Peracchia et al. Biomaterials. 1999 Jul.

Abstract

The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach to investigate the interactions between proteins and particulate systems.

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